Journal: Biochemistry and Biophysics Reports

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

doi: 10.1016/j.bbrep.2026.102568

Figure Lengend Snippet: IFN-γ responsiveness correlates DNA damage and repair responses in NSCLC cell lines (A) Relative cell viability of A549 or PC-9 treated with the indicated concentration of IFN-γ for 24 h are shown. Data are presented as mean ± SD. (B) Reactome analysis of the GSE180942 dataset was performed using differential CRISPR β-scores under IFN-γ treatment (Δβ = PC-9 − A549). Bars indicate the normalized enrichment score (NES) for each pathway indicated. Positive NES denotes pathways whose constituent genes are more essential in A549, whereas negative NES denotes pathways more essential in PC-9 upon IFN-γ treatment.

Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

Techniques: Concentration Assay, CRISPR

Journal: Biochemistry and Biophysics Reports

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

doi: 10.1016/j.bbrep.2026.102568

Figure Lengend Snippet: Inhibition of ATM restores NSCLC cells to IFN-γ by inducing DNA damage response (A) Cell viability of A549 (left panel) or PC-9 (right panel) treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05. (B) Expression of γH2AX and b-Actin (loading control) in A549 (left panel) or PC-9 (right panel) cells treated with IFN-γ (1000 ng/ml) and/or KU-55933 (10 μM) for 24 h are shown.

Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

Techniques: Inhibition, Expressing, Control

Journal: Biochemistry and Biophysics Reports

Article Title: ATM inhibition restores IFN-γ sensitivity and induces ferroptosis in NSCLC via DNA damage response

doi: 10.1016/j.bbrep.2026.102568

Figure Lengend Snippet: Inhibition of ATM in combination with IFN-γ induce ferroptosis in NSCLCs Cell viability of A549 (A) or PC-9 (B) treated with the indicated combination of IFN-γ (1000 ng/ml), KU-55933 (10 μM), Ferrostatin-1 (5 μM), and Liproxstatin-1 (5 μM) for 24 h are shown. Data are presented as mean ± SD. * p < 0.05.

Article Snippet: The human non-small cell lung cancer cell lines PC-9 (kindly gifted from Dr. Kiura, Okayama University, Japan) and A549 (CCL-185, obtained from American Type Culture Collection) were used.

Techniques: Inhibition

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: Internalization of SD-EVLPs and its inhibitory effects on lung cancer cell proliferation (A) Internalization of SD-EVLPs by lung cancer cells; (B, C) CCK-8 assays assessing the effects of SD-EVLPs on lung cancer cell viability; (D–G) EdU assays evaluating the proliferation of A549 and NCI-H1299 cells; (H–I) Colony formation assays assessing the proliferative capacity of A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: CCK-8 Assay, Control

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: SD-EVLPs inhibit migration and invasion of lung cancer cells (A–D) Wound healing assays assessing the effect of SD-EVLPs on the migration of A549 and NCI-H1299 cells; (E, F) Transwell assays evaluating the invasion ability of A549 and NCI-H1299 cells; (G–I) Western blot analysis of E-cadherin and N-cadherin protein expression. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: Migration, Western Blot, Expressing, Control

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: RT-qPCR and Western blot analysis of ferroptosis-related gene expression in lung cancer cells (A, B) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in A549 cells; (C, D) RT-qPCR analysis of GPX4 and SLC7A11 mRNA expression in NCI-H1299 cells; (E–H) Western blot analysis of GPX4 and xCT protein expression in lung cancer cells; (I) TEM assessment of mitochondrial ultrastructure. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: Quantitative RT-PCR, Western Blot, Gene Expression, Expressing, Control

Journal: Frontiers in Oncology

Article Title: Selaginella doederleinii -derived extracellular vesicle-like particles suppress lung cancer with ferroptosis-associated changes and modulation of the FABP4/PPARG/GPX4 axis

doi: 10.3389/fonc.2026.1829211

Figure Lengend Snippet: SD-EVLPs modulate the FABP4/PPARG/GPX4-associated pathway in lung cancer cells (A–D) RT-qPCR analysis of FABP4 and PPARG mRNA expression in A549 and NCI-H1299 cells; (E–H) Western blot analysis of FABP4 and PPARG protein expression in A549 and NCI-H1299 cells. Data are presented as the mean ± SEM, n = 3 independent experiments. Compared with the control group, * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Human lung cancer cell lines A549 (Cat. No.: CL-0016) and NCI-H1299 (Cat. No.: CL-0165) were purchased from Wuhan Procell Life Science & Technology Co., Ltd. Four-week-old male BALB/c nude mice (SPF grade) were purchased from Hunan Silaike Jingda Laboratory Animal Co., Ltd. (Changsha, China), with the Animal Production License No.: SCXK (Xiang) 2021-0002.

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control